VIVALDI - Veterinary Validation of Point-of-Care Detection Instrument

In the VIVALDI project the consortium will validate new equipment (the VETPOD platform) for rapid on-site detection of zoonotic pathogens in industrial food and animal production chains.

The coordinator Technical University of Denmark (DTU) has developed the VETPOD platform based on Loop mediated isothermal amplification (LAMP) technology and optical read-out to a user interface, with disposable plastic cartridges (Lab-on-Chip, LOC) that can be adapted to an infinite number of assays for almost all pathogens.

We have a portable LOC system with optical detection: a system with polymeric chip made by injection moulded with multiple (8-32) chambers suitable for rapid online or on site detection of pathogens. The polymer chip with multiple chambers is able to perform LAMP to detect different pathogens at species level from multiple (8-30) samples within 30-60 min.

We want to validate the VETPOD platform for three important zoonotic pathogens: Avian Influenza Virus (AIV) or Highly Pathogenic Avian Influenza Virus (HPAIV), Salmonellaspp. and Campylobacter spp. For HPAIV this will include identification of H types (H5 and H7), for Salmonella the identification of the most important serovars S. Enteritidis, S. Typhimurium and S. Dublin, and for Campylobacter species identification of C. jejuni and C.coli.

The validation includes two stages:

1) Validation by national reference laboratories in DK, SE, IT and FR. Each NRL will involve 10 external labs for ring trials.

2) End-user validation at private labs (SMEs) in DE and IT.

The equipment provider will prepare a business plan for sale of the VETPOD system.

The private labs will prepare business plans for using the VETPOD platform for at site animal health detection as well as for online detection of zoonotic pathogens in food and animal production chains.

 

Project No: 773422

Duration: 36 months (January 2018 - January 2021)

EU

Work Packages

WP1: Project management, Professor Anders Wolff, DTU Bioengineering
WP2: Providing VETPOD equipment for validation, Professor Anders Wolff, DTU Bioengineering
WP3: Validation of VETPOD for detection of AIV, ANSES
WP4: Validation of VETPOD for detection of Salmonella, National Food Institute
WP5: Validation of VETPOD for detection of Campylobacter, SVA
WP6: Implementation of VETPOD for monitoring of animal health, Acel & Parma
WP7: Implementation of VETPOD for monitoring of food safety, QLAB
WP8: Business plan and market uptake, DNA Diagnostic
WP9: Dissemination and communication, Professor Anders Wolff, DTU Bioengineering
WP10: Ethics, Professor Anders Wolff, DTU Bioengineering.

https://www.vivaldi-ia.eu/research
20 SEPTEMBER 2021